As in the second edition, the third edition of Handbook of Practical Immunohistochemistry – Frequently Asked Questions is written in a question and answer (Q&A) format and intended to be a practical, user-friendly, quick reference for information related to using the most up-to-date immunohistochemistry and in situ hybridization in clinical diagnosis. The new edition demonstrates a significant revision and improvement over the second edition. Five new chapters have been added that cover the following: 1) Immunohistochemistry: Leica’s perspective; 2) Immunohistochemistry: Maixin perspective; 3) RNA in situ hybridization: Applications in anatomic pathology; 4) Applications of rapid immunohistochemistry on frozen tissue sections during intraoperative pathologic diagnosis; and 5) Cutaneous lymphomas. In addition to extensive additions and changes, over 150 new questions and answers were added throughout this new edition. All chapters have been updated to include relevant new questions, new markers, more refined IHC panels, representative pictures, and current references. An extensive set of high-quality color pictures and diagnostic algorithms, if available, is included in each chapter to illustrate some of the key antibodies, including many recently discovered and substantiated antibodies used in that chapter.
Written by experts in the field, Handbook of Practical Immunohistochemistry Third Edition is a comprehensive and practical guide for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
Author(s): Fan Lin, Jeffrey W. Prichard, Haiyan Liu, Myra L. Wilkerson
Edition: 3
Publisher: Springer
Year: 2022
Language: English
Pages: 1043
City: Cham
Preface to the Third Edition
Acknowledgments
Contents
Contributors
Chapter 1: Immunohistochemistry Quality Management and Regulation
Frequently Asked Questions
IHC Quality Management
IHC Laboratory Regulation
References
Chapter 2: Standardization of Diagnostic Immunohistochemistry
Frequently Asked Questions
References
Chapter 3: Automated Immunohistochemistry Overview
Frequently Asked Questions
References
Chapter 4: Immunohistochemistry: An Agilent Perspective
Frequently Asked Questions
References
Chapter 5: The Leica Biosystems Perspective: From Excision to Imaging—Every Step Is Critical
Frequently Asked Questions
References
Chapter 6: Immunohistochemistry: Maixin Perspective
Frequently Asked Questions
References
Chapter 7: Immunohistochemistry: Roche Tissue Diagnostics Perspective
Frequently Asked Questions
References
Chapter 8: Applications of Rapid Immunohistochemistry on Frozen Tissue Sections During Intraoperative Pathological Diagnoses
Frequently Asked Questions
Rapid Immunostains for Melanoma
References
References of Rapid Direct IHC
References for Applications of Rapid Direct IHC for Mohs Micrographic Surgery
SCC and P63/P40 Cytokeratin Refs
References on ck7 Extramammary Paget’s
References on Immunostains for Nonmelanoma Skin Cancer in Mohs
References on DFSP
References on Mohs for Melanoma
References on SOX10
References on MITF
References on Modified DAB
References on Merkel Cell Carcinoma
References for Applications of Rapid Direct IHC for Diagnosis of Breast Lesions
References for Rapid Direct Immunocytochemistry (ICC)
Chapter 9: RNA In Situ Hybridization: Applications in Anatomic Pathology
Frequently Asked Questions (FAQs)
Part 1 – RNA ISH Technology
Part 2 – HPV Detection by RNAscope RNA ISH
Part 3 – Other Applications of RNAscope
Tumor Characterization
Gene Fusion/Amplification
Hematopathology
Other Infectious Agents
Part 4 – Future Directions
References
Chapter 10: Overview of Immunohistochemistry Assessment of Cancer-Related Predictive Biomarkers and Common Genetic Alterations
Frequently Asked Questions
Practical Aspects of PD-L1 and Other Commonly Used Predictive IHC Testing
Role of Mismatch Repair Protein (MMR) IHC for Microsatellite Instability Detection and Universal Lynch Syndrome Screening
Basic Mechanisms and Successful Examples of Integrating IHC into Molecular Pathology
Role of IHC in Detection of NTRK Fusion and Practical Aspects
References
Chapter 11: Tissue Microarray
Frequently Asked Questions
Resources for TMA
References
Chapter 12: Unknown Primary/Undifferentiated Neoplasms
Frequently Asked Questions
Differential Diagnosis
Important Diagnostic Markers and Pitfalls
Diagnostic Panel
Differential Diagnosis: Lung Versus Other Primaries
Breast Versus Other Primaries
Renal Cell Carcinoma Versus Other Primaries
Colorectal Carcinoma Versus Other Primaries
Pancreatic/Biliary Tract Adenocarcinoma Versus Other Primaries
Urothelial Carcinoma Versus Other Primaries
Endometrial Adenocarcinoma Versus Other Primaries
Ovarian Serous Carcinoma Versus Other Primaries
Other Differential Diagnosis, Especially for Cytologic Specimens on Cell Block Preparation
References
Chapter 13: Exfoliative Cytology and Effusions
Frequently Asked Questions
Why Use Cellblock Preparations for Immunohistochemical Analysis?
How Is the Cellblock Prepared?
References
Chapter 14: Breast
Frequently Asked Questions
References
Chapter 15: Predictive Biomarkers in Breast Cancer: ER, PR, and HER-2/NEU
Frequently Asked Questions
References
Chapter 16: Central Nerve System
Markers for Tumors in CNS
Markers for Differential Diagnosis in
Markers for Non-Neoplastic Lesions in CNS
References
Chapter 17: Thyroid, Parathyroid, and Adrenal Glands
What Is the Utility of BRAF Mutation-Specific Antibody in Diagnos
Differential Diagnosis
Parathyroid Gland
Adrenal Glands
References
Chapter 18: Salivary Glands and Head and Neck
Frequently Asked Questions
Salivary Glands
Tumors of the Larynx, Nasopharynx, and Oropharynx
Nose and Nasal Sinus
Middle Ear
Jaw
References
Chapter 19: Pleuropulmonary and Mediastinal Neoplasms
Frequently Asked Questions
Pulmonary Tumors
Differential Diagnosis
Pleural Tumors
Mediastinal Tumors
References
Chapter 20: Uterus
Frequently Asked Questions
References
Chapter 21: Ovary
Frequently Asked Questions
References
Chapter 22: Prostate Gland
Frequently Asked Questions
Differential Diagnosis
References
Chapter 23: Urinary Bladder and Urachus
Frequently Asked Questions
Bibliography
Chapter 24: Kidney
Frequently Asked Questions
References
Chapter 25: Testis and Paratesticular Tissues
Frequently Asked Questions
Bibliography
Chapter 26: Pancreas and Ampulla
References
Chapter 27: Liver, Bile Ducts, and Gallbladder
Frequently Asked Questions
References
Chapter 28: Upper Gastrointestinal Tract
References
Chapter 29: Lower Gastrointestinal Tract and Microsatellite Instability (MSI)
Frequently Asked Questions
References
Chapter 30: Soft Tissue and Bone Tumors
Frequently Asked Questions
References
Chapter 31: Lymph Node
Frequently Asked Questions
Mature B-Cell Neoplasms
Mature T- and NK-Cell Neoplasms
Hodgkin Lymphoma
Immunodeficiency-Associated Lymphoproliferative Disorders
Histiocytic and Dendritic Cell Neoplasms
References
Chapter 32: Bone Marrow
Frequently Asked Questions
References
Chapter 33: Cutaneous Lymphomas
Frequently Asked Questions
Markers for Specific Presentations of Cutaneous T-Cell Infiltration
Cutaneous B-Cell Lymphomas
References
Chapter 34: Infectious Diseases
What Tests Are Available to Detect Bacteria?
Bacillus anthracis
Bartonella henselae
Brucella melitensis
Chlamydia
Erythrasma
Francisella tularensis
Helicobacter pylori
Leptospirosis
Lyme Disease
Meningococci
Pitted Keratolysis
Pseudomonas aeruginosa
Rickettsia Rickettsii/Rocky Mountain Spotted Fever (RMSF)
Staphylococcus Aureus
Treponema pallidum
Yersinia pestis
How Should I Incorporate These Tests Into My Practice?
What Tests Are Available to Detect Mycobacteria?
Anti- bacille Calmette-Guerin (BCG) Antibody Immunostain for Acid Fast Bacteria and Fungi
Mycobacterium avium subsp. Paratuberculosis
Mycobacterium Leprae
Mycobacterium tuberculosis and M. bovis
How Should I Incorporate These Tests Into My Practice?
What Tests Are Available to Detect Viruses?
Cytomegalovirus (CMV)
Enterovirus Infection
Epstein-Barr Virus (EBV)
Hepatitis C
Human Herpes Virus Type 6 (HHV-6)
Human T-Cell Lymphotropic Virus 1 (HTLV-1)
Human Herpes Virus Type 8 (HHV-8)
Human Papillomavirus (HPV)
HSV
Influenza A Virus
Ljungan Virus
Measles (Rubeola)
Parvovirus B19
Powassan Virus
Rabies
Small Pox (Variola)
Varicella-Zoster Virus (VZV)
Viral Hemorrhagic Fevers
West Nile Virus (WNV)
Zika
How Should I Incorporate These Tests Into My Practice?
What Tests Are Available to Detect Fungal and Protozoan Pathogens?
Anti-Bacille Calmette-Guerin (BCG) Antibody Immunostain
Aspergillus species
Balamuthia Mandrillaris
Blastomyces Dermatitidis, Coccidioides Immitis, Cryptococcus Neoformans, and Histoplasma Capsulatum
Chagas Disease
Pneumocystis Carinii
Leshmaniasis
How Should I Incorporate These Tests Into My Practice?
References
Chapter 35: Skin
Markers for Differential Diagnosis
References
Chapter 36: Application of Direct Immunofluorescence for Skin and Mucosal Biopsies: A Practical Review
Frequently Asked Questions
References
Chapter 37: Application of Fluorescent In Situ Hybridization (FISH) in Surgical and Cytologic Specimens (Solid Tumors, Hematopoietic Tumors, Urine, Bile Duct Brushing, and Bronchoscopy)
Frequently Asked Questions
FISH Overview
Hematopoietic Tumor
Chronic Lymphocytic Leukemia (CLL)—Modern Diagnostic Workup
Multiple Myeloma (MM)
Myelodysplastic Syndrome (MDS)
Acute Myeloid Leukemia (AML)
Precursor Lymphoid Neoplasms
Chronic Myelogenous Leukemia (CML)
Lymphoma
UroVysion
FISH in Bile Duct Brushing Specimens
FISH In Bronchoscopic Specimens
FISH in the Diagnosis of Soft Tissue Neoplasms
ALK FISH
ROS1 SureFISH
1p/19q SureFISH
HER2 IQFISH
References
Appendix A: Geisinger IHC Antibody Information and Staining Protocols Using Ventana Benchmark/Leica Bond III Platform
Index
