Author(s): Teledyne Isco, Inc.
Edition: 4th Edition
Publisher: Teledyne Isco, Inc.
Year: 0
Language: English
Pages: 162
Contents......Page 4
Chromatographic Purification in Organic Chemistry......Page 12
Compound Solubility......Page 15
Mobile Phase......Page 16
Mobile Phase Modifiers......Page 18
Retention Factor and Column Volumes......Page 19
Method Development Using TLC......Page 21
TLC and Mobile Phase Techniques......Page 24
Isocratic Elution......Page 25
Gradient Elution......Page 28
Stepped Gradient......Page 29
Linear Gradient......Page 30
Mixed Gradients......Page 32
Loading Capacity of Column......Page 35
Particle Shape......Page 37
Particle Size......Page 38
Automated Chromatography......Page 39
Manual Glass Column Chromatography......Page 46
Benefits of Automation......Page 48
Manually-packed Columns......Page 49
Pre-packed Columns......Page 51
TLC Plates......Page 53
Column Stacking......Page 55
Higher Resolution with small spherical media......Page 59
Improved load capacity......Page 61
Faster purifications......Page 63
Normal Phase Silica......Page 65
Reversed Phase Silica......Page 66
Thin Layer Chromatography Plates......Page 68
Using HPLC Systems to Generate Flash Methods......Page 69
Using the Flash Instrument for Method Development......Page 70
Column Care......Page 72
Solvent Modifiers......Page 73
Low-solubility Polar Heterocycles......Page 75
Primary Amines......Page 76
Carbohydrates......Page 77
Peptides......Page 78
Carboxylic Acids......Page 79
Ionic Compounds......Page 80
RediSep Rf Gold High Performance C18 Columns......Page 81
Storage of the column after use in high pH......Page 84
Specialty Media......Page 86
Amine......Page 87
Basic Alumina......Page 92
Neutral Alumina......Page 95
Cyano......Page 98
Diol......Page 100
SCX......Page 104
SAX......Page 107
Natural Products......Page 111
Cytotoxic Constituents from Butea superba......Page 113
Alkaloids of Banisteria caapi......Page 114
Advanced Solvent Strategies......Page 116
UV Detection......Page 122
Detection with UV-Vis......Page 123
All-Wavelength Detection......Page 124
Example of unknown spectrum......Page 125
Solvent spectrum overlaps compound......Page 127
Sample overloads detector......Page 128
Other Detectors......Page 129
Media Selection......Page 130
Wavelength Selection......Page 145
Elementary theory......Page 151
Application......Page 153
Troubleshooting......Page 156
