American Type Culture Collection, 2013.
This guide contains general technical information for working with animal cells in culture, including media, subculturing, cryopreservation and contaminationGetting Started with an ATCC Cell Line
Product Sheet
Preparation of Medium
Initiating Frozen Cultures
Processing Flask Cultures
Cell Growth and Propagation
Passage Number and Population Doubling
Level
Adapting to a New Medium or Serum
Temperature
Examination of Cultures
Cell Counting
Cell Viability
Subculturing Monolayer Cells
Monolayer Subculturing
Troubleshooting Monolayer Cell Subculturing
Suspension Cells
Suspension Cell Subculturing
Troubleshooting Suspension Cell Subculturing
Adapting a Monolayer Cell Line to Grow in
Suspension
Complete Growth Media
Cell Culture Media
Media Formulations
Media Ingredients
Media Supplements
Osmolality
Antibiotics and Antimycotics
Animal Sera
Culture Vessels and Surfaces
Vessels
Surface Coatings and Feeder Cells
Cryopreservation
Overview
Freeze Medium
Equipment
Liquid Nitrogen Freezer Storage
Cryopreservation Procedure
Recovery of Cryopreserved Cells
Contamination and Biosafety
Check for Microbial Contamination
Mycoplasma Contamination
Treating for Microbiobial Contamination
Cellular Cross-Contamination
Biosafety
Glossary
References
ATCC Media, Sera, and Reagents
